Translational_Unit
ComD

Part:BBa_K316015:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-20)


ComD receptor for CSP-1 (S. pneumoniae), phosphorylates ComE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1363
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains a synthetic RBS optimised for high expression in B subtilis, calculated using RBS calculator [ link ]


Source

Streptococcus pneumoniae, AAC44896.1, codon optimised for expression in B.subtilis

References